Intact Mass

Intact mass can give indications not only of relative abundance of glycoforms, but also stability as degraded mAbs will not give good charge envelope by ESI-MS. Intact Mass with a high resolution MS to identify PTMs, especially relative abundance of glycoforms, combines extremely well with the fast run times and tight peak shapes provided by the bioZen Intact C4 and XB-C8.

Intact Mass of NIST mAb using a bioZen Intact C4 and SCIEX® X500B See Conditions

TIC

UV

Spectra NIST mAb

Deconvoluted Spectra NIST mAb